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51.
Mobbs D Petrovic P Marchant JL Hassabis D Weiskopf N Seymour B Dolan RJ Frith CD 《Science (New York, N.Y.)》2007,317(5841):1079-1083
Humans, like other animals, alter their behavior depending on whether a threat is close or distant. We investigated spatial imminence of threat by developing an active avoidance paradigm in which volunteers were pursued through a maze by a virtual predator endowed with an ability to chase, capture, and inflict pain. Using functional magnetic resonance imaging, we found that as the virtual predator grew closer, brain activity shifted from the ventromedial prefrontal cortex to the periaqueductal gray. This shift showed maximal expression when a high degree of pain was anticipated. Moreover, imminence-driven periaqueductal gray activity correlated with increased subjective degree of dread and decreased confidence of escape. Our findings cast light on the neural dynamics of threat anticipation and have implications for the neurobiology of human anxiety-related disorders. 相似文献
52.
Identification of the cystic fibrosis gene: chromosome walking and jumping 总被引:253,自引:0,他引:253
J M Rommens M C Iannuzzi B Kerem M L Drumm G Melmer M Dean R Rozmahel J L Cole D Kennedy N Hidaka 《Science (New York, N.Y.)》1989,245(4922):1059-1065
An understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA. 相似文献
53.
东北内蒙主要用材树种的种源选择 总被引:1,自引:0,他引:1
以长白落叶松、兴安落叶松、樟子松、红松、红皮云杉、胡桃楸、水曲柳7个主要用材树种的种源选择进行了全面地、系统地、综合地分析与总结。主要对各树种的早期选择,种源遗传稳定性研究及最佳种源选择等3个方面进行研究。分析结果:种源选择不仅为各造林地区提供了最佳种源,而且也为今后各树种的遗传改良、种子调拔、良种基地建设提供了可靠的依据。 相似文献
54.
Krause O Rieke GH Birkmann SM Le Floc'h E Gordon KD Egami E Bieging J Hughes JP Young ET Hinz JL Quanz SP Hines DC 《Science (New York, N.Y.)》2005,308(5728):1604-1606
Two images of Cassiopeia A obtained at 24 micrometers with the Spitzer Space Telescope over a 1-year time interval show moving structures outside the shell of the supernova remnant to a distance of more than 20 arc minutes. Individual features exhibit apparent motions of 10 to 20 arc seconds per year, independently confirmed by near-infrared observations. The observed tangential velocities are at roughly the speed of light. It is likely that the moving structures are infrared echoes, in which interstellar dust is heated by the explosion and by flares from the compact object near the center of the remnant. 相似文献
55.
Unwinding of duplex DNA from the SV40 origin of replication by T antigen 总被引:49,自引:0,他引:49
The T antigen specified by SV40 virus is the only viral-encoded protein required for replication of SV40 DNA. T antigen has two activities that appear to be essential for viral DNA replication: specific binding to duplex DNA at the origin of replication and helicase activity that unwinds the two DNA strands. As judged by electron microscopy, DNA unwinding is initiated at the origin of replication and proceeds bidirectionally. Either linear or circular DNA molecules containing the origin of replication are effective substrates; with closed circular DNA, a topoisomerase capable of removing positive superhelical turns is required for an efficient reaction. Presence of an origin sequence on duplex DNA and a single-strand DNA-binding protein appear to be the only requirements for T antigen to catalyze unwinding. This reaction mediated by T antigen defines a likely pathway to precise initiation of DNA replication: (i) the sequence-specific binding activity locates the origin sequence, (ii) the duplex DNA is unwound at this site, and (iii) the DNA polymerase and primase begin DNA replication. A similar pathway has been inferred for the localized initiation of DNA replication by bacteriophage lambda and by Escherichia coli in which a sequence-specific binding protein locates the origin and directs the DnaB helicase to this site. Observations with the SV40 system indicate that localized initiation of duplex DNA replication may be similar for prokaryotes and eukaryotes. 相似文献
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Protein O-mannosylation is an essential and evolutionarily conserved post-translational modification among eukaryotes. This form of protein modification is also described in Mycobacterium tuberculosis; however, the mechanism of mannoprotein assembly remains unclear. Evaluation of differentially translocated chimeric proteins and mass spectrometry to monitor glycosylation demonstrated that specific translocation processes were required for protein O-mannosylation in M. tuberculosis. Additionally, Rv1002c, a M. tuberculosis membrane protein homolog of eukaryotic protein mannosyltransferases, was shown to catalyze the initial step of protein mannosylation. Thus, the process of protein mannosylation is conserved between M. tuberculosis and eukaryotic organisms. 相似文献
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柑橘是是广西的重要果树品种,在水果产业中占有重要地位。通过对广西柑橘业的现状剖析,从自然资源、品种资源、社会资源、季节、成本、质量、区位等方面提出广西发展柑橘的优势,并对这些优势的利用及实现措施进行探讨,将有利于做强做大广西的柑橘产业。 相似文献